Timothy Okitsu

Analysis of Naturally Arising Simple Deletions in Single Human Colon Crypts with Age

The paper analyzes simple deletions in single human colon crypts across age, finding most are due to strand slippage and NHEJ, with age-related accumulation and radiation effects.

Mutation count and mutation profile analyses of single-nucleotide variants in single human colon crypts

The paper analyzes mutation counts and profiles in single human colon crypts to assess somatic mutation rates and their implications for aging and disease.

Fine Structural Features of Complex InDels and NHEJ Repair at Naturally Occurring Damage Sites in Normal Human Colon Crypts

The paper identifies complex indels and NHEJ repair in human colon crypt stem cells using whole-genome sequencing to study DNA damage repair in a physiologically relevant context.

High-depth Whole Genome Sequencing of Single Human Colon Crypts Uncovers New View on Crypt Clonality

This study uses high-depth whole genome sequencing of single colon crypts to show they are often polyclonal, revealing cell-to-cell heterogeneity previously unattainable with lower sensitivity methods.

Analysis of Naturally Occurring Somatic Insertions in the Human Genome

The paper analyzes naturally occurring somatic insertions in the human genome using whole-genome sequencing, finding replication slippage as a dominant mechanism for these events.