Single Cell Transcriptomics-informed Induced Pluripotent Stem Cells Differentiation to Tenogenic Lineage
Angela Papalamprou, Victoria Yu, Wensen Jiang, Julia Sheyn, Tina Stefanovic, Angel Chen, Chloe M. Castaneda, Melissa Chavez, Dmitriy Sheyn
This study differentiates human iPSCs to the syndetome stage using chemically defined media and small molecules informed by single-cell RNA-sequencing, revealing Wnt pathway involvement in off-target neural differentiation and improved syndetome induction with a WNT inhibitor.
eLife · Apr 24, 2026Peer-reviewedDevelopmental Biology