Acid cleavable biotin-alkyne improves sensitivity for direct detection of biotin labeled peptides in BONCAT analysis.
Daniel B. McClatchy, Patrick T. Garrett, Titus Jung, Jolene K. Diedrich, John R. Yates
This study compares cleavable and uncleavable biotin-alkyne labels in BONCAT analysis, finding that cleavable labels improve protein identification and quantification.
Journal of Proteome Research · Nov 23, 2024Peer-reviewedBiochemistry