Megabodies expand the nanobody toolkit for protein structure determination by single-particle cryo-EM
T. Uchanski, S. Masiulis, B. Fischer, V. Kalichuk, A. Wohlkonig, T. Zogg, H. Remaut, W. Vranken, A. R. Aricescu, E. Pardon, J. Steyaert
The paper introduces megabodies, engineered nanobodies that improve cryo-EM resolution by addressing particle size and orientation issues, and demonstrates their application in solving a membrane protein structure.
bioRxiv · Oct 22, 2019Biochemistry