Phosphorothioate-modified DNA oligonucleotides inactivate CRISPR-Cpf1 mediated genome editing
Bin Li, Chunxi Zeng, Wenqing Li, Xinfu Zhang, Xiao Luo, Weiyu Zhao, Chengxiang Zhang, Yizhou Dong
This study shows that phosphorothioate-modified DNA oligonucleotides block CRISPR-Cpf1 mediated gene editing by inhibiting Cpf1-crRNA complex binding to target DNA.
bioRxiv · Feb 6, 2018 · PreprintBioengineering