Functionalizing cell-free systems with CRISPR-associated proteins: Application to RNA-based circuit engineering
François-Xavier Lehr, Alina Kuzembayeva, Megan E. Bailey, Werner Kleindienst, Johannes Kabisch, Heinz Koeppl
This paper demonstrates the integration of CRISPR-associated proteins into cell-free systems to enhance RNA-based circuit prototyping, showing improved dynamic range and reduced leakage in logic gates.
ACS Synthetic Biology · Aug 12, 2021Peer-reviewedSynthetic Biology