A Coma Pattern-Based Autofocusing Method Resolves Bacterial Cold Shock Response at Single-Cell Level
Sihong Li, Zhixin Ma, Yue Yu, Jinjuan Wang, Yaxin Shen, Xiaodong Cui, Xiongfei Fu, Shuqiang Huang
This paper introduces LUNA, an autofocusing method that enables high-precision single-cell imaging of bacterial cold shock response, revealing three-phase adaptation dynamics and size regulation mechanisms.
eLife · Jul 6, 2026Peer-reviewedMicrobiology