Isolation of secreted proteins from Drosophila ovaries and embryos through in vivo BirA-mediated biotinylation
Leslie M. Stevens, Yuan Zhang, Yuri Volnov, Geng Chen, David S. Stein
The paper describes a modified BirA protein that enables biotinylation of secreted proteins in Drosophila, allowing their isolation from ovaries and embryos.
PLoS ONE · Oct 28, 2019Peer-reviewedMolecular Biology