Author

Z. Daniloski

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Biology Disciplines

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Bioengineering· 1

Signature Assays, Organisms & Methods

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Assay:CRISPR screen· 1 Approach:experimental assay· 1 Research Focus:perturbation response prediction· 1

Frequent Co-Authors

D. McKenzie· 1 shared H.-H. Wessels· 1 shared M. Legut· 1 shared N. E. Sanjana· 1 shared X. Guo· 1 shared X. Xue· 1 shared

Activity by Year

2020-2020 · peak 1/yr

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6 top collaborators · 15 mutual co-author links

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1 shared paperBioengineering

D. McKenzie

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1 paper

Recent·Most cited

High-throughput screens of PAM-flexible Cas9 variants for gene knock-out and transcriptional modulation

M. Legut, Z. Daniloski, X. Xue, D. McKenzie, X. Guo, H.-H. Wessels, N. E. Sanjana

The paper compares PAM-flexible Cas9 variants (xCas9 and Cas9-NG) and wild-type Cas9 across gene knock-out, activation, and suppression using a high-throughput screen, finding that PAM flexibility reduces targeting efficiency and that Cas9-NG outperforms xCas9.

bioRxiv · Jan 22, 2020Bioengineering