Expression of a recombinant, 4’-Phosphopantetheinylated, active M. tuberculosis Fatty acid Synthase I in E. coli
S. Baron, Y. Peleg, J. Grunwald, D. Morgenstern, N. Elad, M. Peretz, S. Albeck, Y. Levin, J. T. Welch, K. A. Deweerd, A. Schwartz, Y. Burstein, R. Diskin, Z. Shakked, O. Zimhony
This paper describes the expression and activation of Mtb FAS I in E. coli using AcpS, confirming its biochemical activity and structural properties.
bioRxiv · Aug 28, 2018Microbiology