Florentine U. Rutaganira

This paper presents an in-house electroporation buffer for choanoflagellates that achieves transfection efficiency comparable to proprietary buffers, increasing accessibility for genetic research.

The paper examines two new loricate choanoflagellate strains that show morphological discrepancies with their genetic relatives, challenging current taxonomic classifications based on morphology.

This study identifies glycerol as a more effective cryoprotective agent than DMSO for preserving Salpingoeca rosetta and its co-cultured bacteria, recommending 15% glycerol and liquid nitrogen for storage.