BioinformaticsPLoS ONEPeer-reviewed
SARS-CoV-2 sequencing artifacts associated with targeted PCR enrichment and read mapping
Kirsten Ellegaard, Vithiagaran Gunalan, Raphael Niklaus Sieber…·Marc Stegger
This study identifies sequencing artifacts in SARS-CoV-2 genome sequencing caused by PCR primers and reference genome choices, showing how they lead to ambiguous base calls and missed mutations.