Jessie J.‐Y. Chang

This study uses long-read RNA sequencing to analyze the transcriptomic response to interferon and influenza infection in ferrets, identifying ISGs and novel transcripts.

This study uses CRISPR-Cas9 to enrich specific transcripts for long-read sequencing, enabling efficient and accurate isoform discovery and low abundance transcript detection.

The paper benchmarks poly(A) tail-length estimation tools using synthetic RNA standards, finding all tools accurate within 12% and recommending Dorado for its speed and ease of use.

The paper compares Nanopore direct RNA-seq and Illumina cDNA-seq in sepsis patients, finding strong correlation and identifying post- and co-transcriptional biomarkers.