Molecular BiologybioRxivPreprint
Easy multiple sequential CRISPR/Cas9 knockouts in cell lines using a Cre/LoxP re-cyclable vector
Dong Li, Nima Etemadi, David Lawrence Vaux
The paper describes a method to sequentially knockout multiple genes in cell lines using a CRISPR/Cas9 lentiviral vector with Cre/LoxP sites, enabling repeated use of the same vector for multiple edits.
DOI 10.1101/2021.02.12.431046