Laura De Clerck

The paper provides a roadmap for selecting a bottom-up mass spectrometry protocol for histone analysis by comparing four methods and introducing a new enzyme.

The paper removes the dependency on DDA libraries in DIA by using predicted spectral libraries, making the workflow deterministic and improving standardization in clinical proteomics.

The paper introduces ion-networks, a data format for DIA MS that captures full data integrity by representing reproducible fragment ions and their consistent co-elution across runs.