CRISPR-based tools for genetic manipulation in pathogenic Sporothrix species
Rémi Hatinguais, Ian Leaves, Gordon D. Brown, Alistair J. P. Brown, Matthias Brock, Roberta Peres da Silva
This paper develops a CRISPR/Cas9-based transformation protocol for targeted gene manipulation in Sporothrix species, enhancing gene deletion efficiency by deleting the KU80 homologue.
bioRxiv · Nov 15, 2022 · PreprintMolecular Biology