Directed evolution of colE1 plasmid replication compatibility: a fast tractable tunable model for investigating biological orthogonality.
Santiago Chaillou, Pinelopi Eleftheria Stamou, Leticia L. Torres, Ana B. Riesco, Warren Hazelton, Vitor Bernardes Pinheiro
The paper engineers compatible ColE1 plasmid origins through directed evolution, showing compatibility depends on complex interactions beyond simple sequence divergence.
Nucleic Acids Research · Aug 24, 2022Peer-reviewedSynthetic Biology