Vitor Bernardes Pinheiro

This paper develops a modular click-chemistry strategy to modify CRISPR RNPs without disrupting their formation, enhancing DNA accessibility and enabling genome imaging.

This study optimizes directed evolution protocols by analyzing selection conditions and sequencing coverage to improve efficiency and accuracy in identifying enriched mutants.

The paper engineers compatible ColE1 plasmid origins through directed evolution, showing compatibility depends on complex interactions beyond simple sequence divergence.

Lira provides rotational invariant descriptors for small molecules and protein pockets using real spherical harmonics, enabling efficient comparison and analysis for drug design.

This paper introduces SNAP as a quantitative reporter for bacterial cell display, enabling the development of a versatile platform for engineering low affinity ligands and enzymes.

The paper identifies two new plasmid post-segregational killing mechanisms in E. coli that enhance plasmid stability in both in vitro and in vivo settings.

Darwin Assembly efficiently generates large, high-quality mutagenesis libraries with multiple distant mutations in a single day.

The paper introduces InDel assembly, a DNA platform for generating and analyzing protein loop libraries with varied length and composition, and applies it to engineer TEM-1 β-lactamase loops.