Sean W. J. Prosser

The Metabarcoding Analysis Pipeline (MAP): Simple, accurate, and flexible metabarcoding

The paper introduces MAP, a metabarcoding pipeline that handles long-read data and provides accurate biodiversity estimates, outperforming existing platforms for COI metabarcoding.

The Barcode Inference Pipeline (BIP): From Sequencer Output to DNA Barcodes

The paper introduces BIP, a pipeline for DNA barcode inference from sequencing data, which outperforms existing tools by accurately distinguishing target and non-target sequences.

Performance Test of the QNome Nanopore Sequencer

The paper tests the performance of Qitan Technology's nanopore sequencer, finding it generates accurate DNA barcodes at a lower cost than Oxford Nanopore Technologies' sequencer.

Incidence and attributes of chimeric COI and 18S sequences derived from nematode-infected arthropods

This study examines chimeric sequences in nematode-infected arthropods using nanopore sequencing, finding higher incidence in 18S than COI and linking chimera formation to DNA stability and sequence conservation.

Barcode 100K Specimens: In a Single Nanopore Run

This study demonstrates a cost-effective method for DNA barcoding using Oxford Nanopore Technologies, enabling the processing of 100,000 specimens per flow cell at $0.01 per specimen.

Massive Multiplexing Can Deliver a $1 Test for COVID-19

This paper describes a low-cost diagnostic test for COVID-19 using sequence variation in the envelope protein gene, enabling analysis of 10,000 samples per run with RNA extraction, RT-PCR, and high-throughput sequencing.

A Sequel to Sanger: Amplicon Sequencing That Scales

This paper evaluates SMRT sequencing on the SEQUEL platform for amplicon sequencing, finding it provides more complete coverage and reduces costs compared to Sanger sequencing.