Cas12a cleavage and trimming kinetics reveal mismatches as a strategy to steer editing
Uzair Ahmed, Fausta Michnevičiūtė, Marius Vinogradovas, Eimina Dirvelytė-Valauskė, Urtė Neniškytė, Stephen K. Jones
This study shows that reprogrammed gRNAs with mismatches can shift editing outcomes to improve gene knockout efficiency without reducing overall editing efficiency.
bioRxiv · Sep 22, 2026 · PreprintBiochemistry