Stephen K. Jones

This study shows that reprogrammed gRNAs with mismatches can shift editing outcomes to improve gene knockout efficiency without reducing overall editing efficiency.

This paper introduces a method for efficient, single-step genome editing of bacteriophages using targeted DNA nicking, achieving high recombination rates without reducing phage titers.

This study investigates how DNA supercoiling affects Cas9 off-target cleavage by mapping cleavage rates and sites across different topologies using NucleaSeq and a biophysical model.

The paper introduces CRISPRzip, a mechanistic model that predicts CRISPR-Cas9 activity based on sequence and context, validated by experimental data.

The paper profiles cleavage specificity and kinetics of CRISPR nucleases using NucleaSeq and CHAMP, finding engineered Cas9s show improved specificity while wtCas12a has similar specificity to wtCas9 in vitro.

The paper shows that RNAi-induced heterochromatin spread is stochastic, while a memory element called REIII provides deterministic, high-fidelity spreading in fission yeast.

The paper introduces and validates FREE barcodes, which correct DNA synthesis and sequencing errors, including insertions and deletions, improving accuracy and efficiency in high-throughput sequencing.

This study uses a microfluidic platform to show that fission yeast cells can divide for over 75 generations without showing signs of aging, and their lifespan can be extended by genetic or drug interventions.