Changing the initiation unit of nonribosomal peptide synthetases to access underexplored biosynthetic potential
Xianping Bai, Lin Zhong, Hanna Chen, Yang Liu, Xingxing Shi, Xingyan Wang, Qingsheng Yang, Xiaotong Diao, Dalei Wu, Youming Zhang, Xiaoying Bian
This paper introduces a strategy to activate silent NRPS biosynthetic gene clusters by swapping initiation units, successfully recovering three of six previously silent pathways and identifying five novel lipopeptides.
bioRxiv · May 29, 2025 · PreprintSynthetic Biology