Copy Number Variation-Based Molecular Sexing of Ixodes scapularis and Rhipicephalus microplus Immature Stages Using qPCR and ddPCR Approaches
Sharma Arvind, Benjamin Faustino, Isaac Amankona Hinne, Jakub Wudarski, Saransh Beniwal, Nurul Hamidi, Kyla Ercit, Sanjay Basu Basu, Malimba Lisulo, Mattia Poletto, Kelly Jean Matzen, Monika Gulia-Nuss
The paper develops and validates a copy number variation-based molecular sexing method for immature Ixodes scapularis and Rhipicephalus microplus ticks using qPCR and ddPCR, achieving high accuracy in sex determination.
Ecology and Evolution · Oct 28, 2026Peer-reviewedMolecular Biology