Zhisong Chen

This study benchmarks five loss-of-function perturbation tools for studying noncoding genome function, finding dual-SpCas9 is most effective for targeting CREs and recommending RNA interference for noncoding RNA genes.

The paper benchmarks multiple base editors and develops a model to predict editing efficiency, validating edits for disease-associated variants and identifying cancer-related SNVs.

The paper evaluates CRISPR-Cas13 systems and finds that they have intrinsic RNA targets in mammalian cells, which can affect lentiviral particle production and limit their utility.