Author

Kaiyven Afi Leslie

University of ExeterORCID 0000-0002-1591-0942Unclaimed ORCID profile · follow to get notified of new preprints

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Molecular Biology· 1

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Assay:CRISPR screen· 1 Approach:experimental assay· 1 Organism:human· 1 Research Focus:protein design· 1

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Asami Oguro‐AndoiD· 1 shared Emily-Rose MartiniD· 1 shared Josan G. MartiniD· 1 shared Mark A. RusselliD· 1 shared

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2025-2025 · peak 1/yr

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1 shared paperMolecular Biology

Asami Oguro‐AndoORCID

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1 paper

Recent·Most cited

Molecular BiologybioRxivPreprintOct 23, 2025

Sleeping ORANGE: A Transposase-Enhanced CRISPR Approach to Boost Endogenous Protein Tagging Efficiency

Emily-Rose Martin, Josan G. Martin, Kaiyven Afi Leslie…·Asami Oguro‐Ando

The paper introduces Sleeping ORANGE, a modified CRISPR-Cas9 method that enhances endogenous protein tagging efficiency using a Sleeping Beauty transposase fusion, achieving a 12.85-fold increase in editing efficiency for CYFIP1 tagging.

DOI 10.1101/2025.10.23.684145

humanCRISPR screen

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