Author

Daisuke Arai

Waseda UniversityORCID 0000-0002-1723-5389Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Molecular Biology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 1 Organism:mouse· 1

Frequent Co-Authors

Yoichi NakaoiD· 1 shared

Activity by Year

2021-2021 · peak 1/yr

1 paper

Recent·Most cited

Molecular BiologyScientific ReportsPeer-reviewedSep 13, 2021

Efficient biallelic knock-in in mouse embryonic stem cells by in vivo-linearization of donor and transient inhibition of DNA Polymerase θ/DNA-PK

Daisuke Arai, Yoichi Nakao

This paper improves HDR-mediated knock-in efficiency in mESCs by inhibiting NHEJ/MMEJ and linearizing donor DNA, achieving high biallelic knock-in rates.

26 citations

mouseexperimental assay

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