Single-cell Rapid Capture Hybridization sequencing (scRaCH-seq) to reliably detect isoform usage and coding mutations in targeted genes at a single-cell level
Hongke Peng, Jafar S. Jabbari, Luyi Tian, Changqing Wang, Yupei You, Chong Chyn Chua, Natasha S. Anstee, Noorul Amin, Andrew H. Wei, N. Davidson, Andrew Warwick Roberts, David C.S. Huang, Matthew E. Ritchie, Rachel Thijssen
The paper introduces scRaCH-seq, a method for single-cell long-read sequencing that improves detection of isoform usage and mutations in targeted genes, demonstrating its application in CLL samples.
bioRxiv · Jan 30, 2024Molecular Biology