The paper uses long-read sequencing to generate complete genomes of Mycobacterium tuberculosis complex isolates, revealing increased genomic diversity and improved resolution in transmission analysis.

bioRxiv · Aug 5, 2025 · PreprintGenomics

Genetic diversity within diagnostic sputum samples is mirrored in the culture of Mycobacterium tuberculosis

Carla Mariner-Llicer, Galo Adrián Goig, Manuela Torres‐Puente, Sergo A. Vashakidze, Luis M. Villamayor, Belén Saavedra, Edson Mambuque, Iza Khurtsilava, Zaza Avaliani, Alex Rosenthal, Andrei E. Gabrielian, Marika Shurgaia, Natalia Shubladze, Alberto L. García‐Basteiro, Mariana G. López, Iñaki Comas

This study shows that culturing Mycobacterium tuberculosis from sputum samples accurately mirrors the genetic diversity found in the original clinical samples, with high variant agreement and correlation.

bioRxiv · Jan 30, 2024 · PreprintGenomics

Unravelling the population structure and transmission patterns of Mycobacterium tuberculosis in Mozambique, a high TB/HIV burden country

Belén Saavedra, Mariana G. López, Álvaro Chiner‐Oms, Ana María García García, Irving Cancino‐Muñoz, Manuela Torres‐Puente, Luis M. Villamayor, Carlos Francisco Madrazo-Moya, Edson Mambuque, Guillermo Sequera, Durval Respeito, Silvia Blanco, Orvalho Joaquim Augusto, Elisa López‐Varela, Alberto L. García‐Basteiro, Iñaki Comas

The paper uses whole-genome sequencing to analyze Mycobacterium tuberculosis population structure and transmission patterns in Mozambique, finding recent transmission and endemic clades linked to HIV/TB coinfected patients.

Microbial Genomics · Jul 5, 2022Peer-reviewedGenetic & Genomic Medicine