Author

Nobuhiro Tsutsumi

The University of TokyoORCID 0000-0002-2610-6837Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Organism:Arabidopsis· 3 Approach:experimental assay· 3 Assay:DNA methylation· 2 Research Focus:gene regulatory network inference· 2 Assay:whole-genome sequencing· 2 Research Focus:cell type annotation· 1 Research Focus:chromatin & epigenetic regulation· 1 Approach:statistical model· 1

Frequent Co-Authors

Miki OkunoiD· 3 shared Shin‐ichi ArimuraiD· 3 shared Issei NakazatoiD· 2 shared Takehiko ItohiD· 2 shared Ayako Hosoda· 1 shared Hideki TakanashiiD· 1 shared Nanami KosakaiD· 1 shared Yoshiki Harada· 1 shared

Activity by Year

2024-2026 · peak 1/yr

Co-Author Collaboration Network

9 top collaborators · 26 mutual co-author links

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3 shared papersPlant Biology

Miki OkunoORCID

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3 papers

Recent·Most cited

Chloroplast- and Mitochondrion-Specific Random C-to-T Mutagenesis for Forward Genetics of Organelle Genomes

Nanami Kosaka, Yoshiki Harada, Issei Nakazato, Miki Okuno, Takehiko Itoh, Nobuhiro Tsutsumi, Shin‐ichi Arimura

This paper develops a method to introduce random C-to-T mutations in chloroplast and mitochondrial genomes of Arabidopsis thaliana to enable forward genetics for organelle genome studies.

The Plant Journal · Jun 1, 2026Peer-reviewedPlant Biology

Mitochondrial DNA and 641kb nuclear-mitochondrial DNA in Arabidopsis can be separated by their CpG methylation levels

Yuyang Zhong, Miki Okuno, Nobuhiro Tsutsumi, Shin‐ichi Arimura

The paper separates mitochondrial and NUMT DNA in Arabidopsis by CpG methylation levels, showing mitochondrial DNA has minimal methylation.

PLANT PHYSIOLOGY · Feb 20, 2025Peer-reviewedPlant Biology

TALE-based C-to-T base editor for multiple homologous genes with flexible precision

Ayako Hosoda, Issei Nakazato, Miki Okuno, Takehiko Itoh, Hideki Takanashi, Nobuhiro Tsutsumi, Shin‐ichi Arimura

The paper describes a TALE-based C-to-T base editor for precise genome editing in Arabidopsis, enabling simultaneous editing of homologous genes with reduced off-target effects.

Plant Biotechnology · Jul 1, 2024Peer-reviewedSynthetic Biology