Gianvincenzo Zuccotti

Food-Allergic Children Harbour a Reduced Oral Bacterial Load, Giving Misleading 16S rRNA Amplicon Profiles

The paper reanalyzes oral microbiota data from food-allergic children using absolute abundance instead of relative abundance, revealing reduced bacterial load and altered functional predictions.

A Simple and Cost-Effective Protocol for Enriching 'Ca. Saccharimonadia' from Human Saliva to Enable Efficient Genome Sequencing

This paper develops a cost-effective protocol to enrich 'Ca. Saccharimonadia' from human saliva, enabling high-quality genome sequencing and metagenome-assembled genomes.

Unraveling and quantifying "Candidatus Saccharibacteria": in silico and experimental evaluation of V3-V4 16S rRNA metagenomics and qPCR protocols

The paper evaluates qPCR and 16S metagenomics methods for quantifying 'Candidatus Saccharibacteria', finding that 16S metagenomics better detects its expansion in allergic subjects than qPCR protocols.

Robustness of time domain near infrared spectroscopy to variations in skin pigmentation

This study evaluates the robustness of time domain near infrared spectroscopy to variations in skin pigmentation using tissue phantoms and pediatric volunteers, finding negligible effects on results.

RecA is a reliable marker for bacterial taxonomy, even in the Candidate Phyla Radiation

This study introduces RecA as a reliable genetic marker for bacterial taxonomy, showing it provides accurate taxonomic annotation comparable to multi-gene approaches, especially for the Candidate Phyla Radiation.