Untrimmed ITS2 metabarcode sequences cause artificially reduced abundances of specific fungal taxa
Kathleen E. Kyle, Jonathan L. Klassen
The paper identifies quality biases in ITS2 metabarcoding that reduce specific fungal taxa abundances and recommends sliding window trimming to mitigate these biases.
Applied and Environmental Microbiology · Dec 26, 2024Peer-reviewedMicrobiology