Implementation of an adaptive-optics assisted isoSTED nanoscope
Yang Li, Dong‐Ryoung Lee, Edward S. Allgeyer, Lena K. Schroeder, Shijie Tu, Joerg Bewersdorf, Xiang Hao
This paper describes the construction and alignment of an adaptive-optics assisted isoSTED nanoscope for achieving isotropic sub-50-nm 3D resolution in thick biological samples.
Nature Protocols · May 8, 2026Peer-reviewedBioengineering