Joerg Bewersdorf

SPC-Clean: A napari Plugin for Reducing Speckle and Isolated Pixel Noise in Fluorescence Microscopy Images

SPC-Clean reduces speckle and isolated pixel noise in fluorescence microscopy images using a topology-aware method without altering fluorescence intensities.

Implementation of an adaptive-optics assisted isoSTED nanoscope

This paper describes the construction and alignment of an adaptive-optics assisted isoSTED nanoscope for achieving isotropic sub-50-nm 3D resolution in thick biological samples.

Fluorogenic speed-optimized DNA-PAINT probes enable super-resolution imaging of whole cells

This paper introduces fluorogenic DNA-PAINT probes that improve super-resolution imaging speed and signal-to-background ratio in cells.

All-optical visualization of specific molecules in the ultrastructural context of brain tissue

This paper introduces pan-ExM-t, an all-optical method for visualizing specific proteins in the ultrastructural context of brain tissue, demonstrating its application on various markers in mouse brain sections and neuron cultures.

Renalase activates mitochondrial leak metabolism in response to cellular stress and to repair damage after injury

This paper shows that renalase activates mitochondrial leak metabolism to enhance protein synthesis and protect cells from stress and injury.

Pan-ASLM: a high-resolution and large-field-of-view light sheet microscope for Expansion Microscopy

The paper introduces pan-ASLM, a light sheet microscope optimized for expansion microscopy, achieving higher speed, larger field of view, and better resolution than standard confocal microscopes.

Scalable automated segmentation quantifies mitochondrial proteins and morphology at the nanoscale

This paper introduces MAPS, a deep-learning approach for automated segmentation of mitochondrial ultrastructure in 3D pan-ExM images, enabling high-throughput quantification of morphology and protein localization.

The Golgi Rim is a Precise Tetraplex of Golgin Proteins that Can Self-Assemble into Filamentous Bands

The paper uses super-resolution microscopy to show golgin proteins form a tetraplex at the Golgi rim and self-assemble into filamentous bands, proposing a model for Golgi stack formation.

ATG2A interacts with RAB1A and ARFGAP1 positive membranes during autophagosome biogenesis

This paper identifies RAB1A and ARFGAP1-positive membranes as essential for autophagosome biogenesis, interacting with ATG2A to support lipid transport during autophagosome formation.

A correlative quantitative phase contrast and fluorescence super-resolution microscope for imaging molecules in their cellular context

The paper develops a combined microscope platform that integrates quantitative phase contrast and super-resolution fluorescence imaging for high-sensitivity visualization of sub-cellular structures and molecules in their cellular context.

Single-cell in vivo brain optogenetic stimulation by two-photon excitation fluorescence transfer

This paper introduces a method to enhance two-photon optogenetic stimulation in live mouse brains by using fluorescent molecules to improve opsin activation efficiency.

A stable, high refractive index, switching buffer for super-resolution imaging

The paper develops a stable, high refractive index buffer for dSTORM super-resolution imaging, improving stability, switching speed, contrast, and refractive index matching.

Nano-scale size holes in ER sheets provide an alternative to tubules for highly-curved membranes

The paper identifies dynamic nano-scale holes in ER sheets that act as reservoirs for curvature-stabilizing proteins, enabling ER tubule extension and retraction.