In situ protein crystallography: a single-crystal electron diffraction pipeline for structure determination inside living cells.
Štěpánka Bílá, Dominik Pinkas, Krishna Prasad Khakurel, Juliane Boger, Tomáš Bílý, János Hajdu, Zdeněk Franta, Roman Tůma, Lars Redecke, Vitaly Polovinkin
The paper introduces IncellED, a method combining InCellCryst with 3D electron diffraction to determine high-resolution protein structures from single intracellular crystals.
bioRxiv · Jul 8, 2025 · PreprintBiophysics