Discovery, characterization, and application of chromosomal integration sites in the hyperthermophilic crenarchaeon Sulfolobus islandicus
Aashutosh Girish Boob, Changyi Zhang, Yuwei Pan, Airah Zaidi, Rachel J. Whitaker, Huimin Zhao
This study identifies and characterizes chromosomal integration sites in Sulfolobus islandicus using CRISPR-COPIES and validates their utility for genetic manipulation.
bioRxiv · Mar 16, 2025 · PreprintSynthetic Biology