Aashutosh Girish Boob

Design of diverse, functional mitochondrial targeting sequences across eukaryotic organisms using variational autoencoder

This paper uses a variational autoencoder to design novel mitochondrial targeting sequences across eukaryotic organisms, showing they are functional and diverse.

Discovery, characterization, and application of chromosomal integration sites in the hyperthermophilic crenarchaeon Sulfolobus islandicus

This study identifies and characterizes chromosomal integration sites in Sulfolobus islandicus using CRISPR-COPIES and validates their utility for genetic manipulation.

CRISPR-COPIES: An in silico platform for discovery of neutral integration sites for CRISPR/Cas-facilitated gene integration

CRISPR-COPIES identifies neutral integration sites for CRISPR/Cas-mediated gene insertion in bacterial, fungal, and human genomes using computational methods.

A landing pad system for multicopy gene integration in Issatchenkia orientalis

This paper develops a landing pad system for efficient multicopy gene integration in Issatchenkia orientalis using CRISPR-Cas9, enabling the construction of complex metabolic pathways with high integration efficiency.

PlasmidMaker: a Versatile, Automated, and High Throughput End-to-End Platform for Plasmid Construction

PlasmidMaker automates high-throughput plasmid construction with error-free assembly of complex genetic sequences across multiple species.