Enhancement of RecET-mediated in vivo linear DNA assembly by a xonA mutation
James A. Sawitzke, Nina Costantino, Adriana Castillo Caballero, Ellen Hutchinson, Alessandro Barenghi, Lynn C. Thomason, Donald L. Court
The paper improves in vivo linear DNA assembly efficiency by deleting the xonA gene, which encodes Exonuclease I, enhancing both RecET and lambda Red systems.
PLoS ONE · Apr 3, 2026Peer-reviewedMolecular Biology