Production of cellobiose from ionic liquid-treated cellulose using the highly thermostable cellobiohydrolase HmCel6A-3SNP at 80°C and analysis of enzymatic accessibility to the substrate
Takeshi Ara, Tsutomu Kodaki, Yoichi Ogawa, Tomoya Imai, Satoru Takahashi, Yoshitsugu Hirose, Daisuke Shibata, Toshiyuki Nohira
This study produces cellobiose from ionic liquid-treated cellulose using a thermostable enzyme at 80°C, achieving higher yields and purity through optimized pretreatment and enzyme conditions.
bioRxiv · Jun 3, 2026 · PreprintBiochemistry