Cell-free prediction of protein expression costs for growing cells
Olivier Borkowski, Carlos Bricio, Michela Murgiano, Brooke Rothschild-Mancinelli, Guy-Bart V. Stan, Tom Ellis
The paper predicts the cost of protein expression in E. coli using a cell-free assay and computational model to assess resource consumption and differentiate between gene expression and metabolic pathway burdens.
Nature Communications · Apr 11, 2018Peer-reviewedSynthetic Biology