Brooke Rothschild-Mancinelli

This paper demonstrates the directed evolution of the Saccharomyces cerevisiae mitochondrial translation system as an orthogonal system to the cytosolic TS, revealing functional dependencies and enabling translation engineering.

The paper predicts the cost of protein expression in E. coli using a cell-free assay and computational model to assess resource consumption and differentiate between gene expression and metabolic pathway burdens.