Martijn de Roij

This study shows that MpARF2 forms nanoclusters that enable high-affinity, switch-like, and sequence-specific DNA binding, distinct from monomeric or condensate-based mechanisms.

The paper identifies a biochemical mechanism by which the MpARF1 transcription factor activates gene expression through subnuclear clustering and Mediator complex recruitment, revealing an evolutionary innovation in auxin response.

The paper identifies a conserved residue critical for proteolysis of B-class ARF MpARF2 and shows differential functional impact of degradation in A-class MpARF1.

The paper identifies a minimal region responsible for ARF degradation in Marchantia polymorpha, showing it is critical for development and auxin response, and suggests ARF instability predates the auxin response system.

The paper shows that the ARF-DNA interface is ultra-conserved across plant lineages, explaining the evolutionary stability of auxin signaling.

This study investigates the evolutionary origins and functional diversification of Auxin Response Factors (ARFs) through in silico and in vivo analyses, revealing their shared origin and evolutionary trajectory.