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Inference of CRISPR Edits from Sanger Trace Data
Tim Hsiau, David Conant, Nicholas Rossi…·Rich Stoner
ICE regresses simulated edit traces onto Sanger reads to quantify indels, multi-guide and knock-in edits without tuning, correlating with amplicon sequencing (r2 = 0.93) and TIDE (r2 = 0.99).
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