The interplay of DNA repair context with target sequence predictably biases Cas9-generated mutations
Ananth Pallaseni, Elin Madli Peets, Gareth Girling, Luca Crepaldi, Ivan Kuzmin, Marilin Moor, Núria Muñoz-Subirana, Joost Schimmel, Özdemirhan Serçin, Balca R. Mardin, Marcel Tijsterman, Hedi Peterson, Michael Kosicki, Leopold Parts
The paper investigates how DNA repair genes influence Cas9-induced mutations by analyzing mutational outcomes across 2,838 synthetic targets in mouse cells, revealing gene-specific roles and improving predictive models.
Nature Communications · Nov 26, 2024Peer-reviewedGenetics