DMEM, or Opti-MEM, that is the Question: An Important Consideration for Extracellular Vesicle Isolation and their Downstream Applications.
Nikki Salmond, Jacob Melamed, Sina Halvaei, Karla C. Williams
This study compares EV release and composition in different media, finding that Opti-MEM promotes more EV release and distinct pathway usage compared to EV-DEP DMEM.
bioRxiv · Nov 17, 2025 · PreprintCell Biology