On the path to reference genomes for all biodiversity: lessons learned and laboratory protocols created in the Sanger Tree of Life core laboratory over the first 2000 species
Caroline Howard, Amy Denton, Benjamin G. Jackson, Adam Bates, Jessie Jay, Halyna Yatsenko, Priyanka Sethu Raman, Abitha Thomas, Graeme Oatley, Raquel Juliana Vionette do Amaral, Zeynep Ene Göktan, Juan Pablo Narváez-Gómez, Isabelle Clayton Lucey, Elizabeth Sinclair, Michael Andrew Quail, , Mara Lawniczak
Across 4883 species, standardised high-molecular-weight DNA extraction passed 96% of chordate and 91% of plant species, while fungi failed at 34.2%; ultra-low-input PacBio libraries rescue many low-yield samples.
bioRxiv · Apr 11, 2025 · PreprintMolecular Biology✦ In-depth digest