Single- and duplex TaqMan-quantitative PCR for determining the copy numbers of integrated selection markers during site-specific mutagenesis in Toxoplasma gondii by CRISPR-Cas9
Kai Pascal Alexander Hänggeli, Andrew Frederick Hemphill, Norbert Müller, Bernd Schimanski, Philipp Olias, Joachim Müller, Ghalia Boubaker
The paper develops single and duplex TaqMan qPCR methods to accurately quantify integrated selection markers in Toxoplasma gondii CRISPR-Cas9 knockouts.
bioRxiv · Jun 23, 2022 · PreprintMolecular Biology