Differential laboratory passaging of SARS-CoV-2 viral stocks impacts the in vitro assessment of neutralizing antibodies
Aram Avila-Herrera, Jeffrey A. Kimbrel, Jose Manuel Martí, James B. Thissen, Edwin A. Saada, Tracy Weisenberger, Kathryn T. Arrildt, Brent W. Segelke, Jonathan Allen, Adam T Zemla, Monica K. Borucki
The paper shows that passaging SARS-CoV-2 viral stocks under antiserum selection pressure increases genetic diversity and antibody escape mutations, making them more representative of circulating viruses.
PLoS ONE · Jan 25, 2024Peer-reviewedMolecular Biology