GenomicsJournal of Biological ChemistryPeer-reviewed
A mechanistic study on the tolerance of PAM distal end mismatch by SpCas9
Dhritiman Dey, Rudra Chakravarti, Oindrila Bhattacharjee…·Dipanjan Ghosh
The paper investigates how mismatches at the PAM distal end affect SpCas9 activity through experimental and computational methods, finding that certain mismatches reduce activity due to conformational instability.
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