Purification protocol of hypertrophied hepatic stellate cells for their transcriptomic characterization from CDAHFD mice liver
Marion Heckmann, Nour El Houda Djerir, Pierre‐Henri Commère, Julien Fernandes, Guillaume Sarrabayrouse, Pascal Bigey, Virginie Escriou, Céline Hoffmann
The paper describes a protocol to purify hypertrophied hepatic stellate cells (hypHSCs) from CDAHFD mice and performs bulk RNA-seq to show their distinct molecular signature compared to quiescent HSCs.
bioRxiv · May 19, 2025 · PreprintCell Biology