A FRET assay to quantitate levels of the human β-cardiac myosin interacting heads motif based on its near-atomic resolution structure
Rama Reddy Goluguri, Piyali Guhathakurta, Neha Nandwani, Aminah Dawood, Seiji Yakota, Osha Roopnarine, David Dale Thomas, James A. Spudich, Kathleen M. Ruppel
The paper develops a FRET assay to directly quantify the IHM state of human β-cardiac myosin and shows how mutations and perturbations affect its stability.
bioRxiv · Dec 5, 2024 · PreprintBiophysics